Part:BBa_K2719012:Design

TCD5 coding device with blue chromoprotein

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1257
  

Design Notes

For tenascin was used a blue chromoprotein to improve the way of selecting colonies. For this chromoprotein was used a constitutive promoter and a double terminator. In the other case, for tenascin cassette was used a T7-lac promoter induced by IPTG. Also, it was created to be compatible with E. coli BL21 (DE3) and with pSB1C3 plasmid. (Figure 1)

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Figure 1. Diagram of BBa_K2719012, leptin expression device.

Source

TCD5 coding device with blue chromoprotein